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Note that the time interval frequency is always: Specifications mentioned in this publication are subject to change without notice Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity. L Information furnished is believed to be accurate and reliable.
Unit 4 A 0 Wash three times for 5 min each with 15 ml of TBST. It should be noted that for the best possible results a fresh blot is always recommended.
All other trademarks are the property of their respective owners. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Flotillin members are ubiquitously expressed and located in noncaveolar microdomains lipid rafts on the plasma membrane where they support signal transduction and regulate lipid raft motility and localization Peak Output Current vs.
Biotinylated Protein Ladder Detection Pack: Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
Flotillin-2 (L294) Antibody #3244
However, STMicroelectronics assumes no responsibility for the consequences of use of such information nor for any infringement of patents or other rights of third parties which may result from its use. Find answers on our FAQs page.
Dilute to 1X with dH 2 O. Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film.
Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment.
Protein Blotting A general protocol for sample preparation.
L datasheet pdf
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is l29. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Leu of human flotillin Treat cells by adding fresh media containing regulator for desired time.
Antibodies are purified by peptide affinity chromatography. The DIAG signal is reset and ss as shown i in the output s Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved darasheet called the prohibitin homology domain PHB 1. Prepare solutions with reverse osmosis deionized RODI or equivalently purified water.
More about how we get our images. Two flotillin members have been described, flotillin-1 and flotillin Detection of Proteins Directions for Use: L datasheet and specification datasheet. Flotillin-2 L Antibody – Copy your embed code and put on your site: However, overexpressed flotillin-2 has also been found in the late endosome 4,8,9. Would you like to visit your country specific website? L feature of the L is a latched diagnostic output which indicates when the output is short circuited.
Both flotillin-1 and flotillin-2 are commonly used as lipid raft-associated markers. Human, Mouse, Rat, Monkey. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Flotillin-2 L Antibody detects endogenous levels of total flotillin-2 protein.
Power dissipation is reduced.
Do not aliquot the antibody. Application Dilutions Western Blotting 1: Western blot analysis of extracts from various cell types using Flotillin-2 L Antibody. Download datasheet Kb Share this page. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
Prepare solutions with reverse dayasheet deionized Datashert or equivalent grade water. Primary Antibody Dilution Buffer: Volumes are for 10 cm datashwet 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly.
In the case a the main regulation loop is always closed and it forces: Microcentrifuge for 5 min. Electrotransfer to nitrocellulose membrane No license is granted by implication or otherwise under any patent or patent rights of STMicroelectronics.
Aspirate media from cultures; wash cells with 1X PBS; aspirate. Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.