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It should be noted that peptide 2 corresponds to the caveolin region residues that we have implicated in the direct interaction of caveolin with c-Src using caveolin expressed as a GST fusion protein see Fig. In contrast, recombinant overexpression of wild-type c-Src does not result in efficient cell transformation or increased tyrosine phosphorylation, indicating that the tyrosine kinase activity of c-Src is normally repressed 37 We cannot process a return if you do not email us.
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Delivery times may vary, especially during peak periods and will depend on when your payment clears – opens in a new window or tab. Protein-tyrosine kinases play an essential role in the regulation of cellular growth control and differentiation.
DS 428 DARK BROWN SMOOTH MATTE
Seller information carpartsinmotion GST-caveolin fusion proteins were expressed and purified as we described previously 2425 29109, Within min, this mixture was added to the cells; dishes were gently agitated to ensure uniform mixing. Redeem your points Conditions for uk nectar points – opens in a new window or tab. Report item – opens in a new window or tab. Mutationally activated c-Src has been implicated in the pathogenesis of carcinomas, including breast tumors Please enter up to 7 characters for the postcode.
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Thus, in vitro auto-phosphorylation of c-Src serves as a measure of its auto-activation. Also, the site of caveolin phosphorylation by c-Src occurs at a single tyrosine residue tyrosine 14; Ref.
Effects of caveolin peptides on the auto-activation of Fyn, a closely related Src family tyrosine kinase. First, it is required to form multivalent homo-oligomers of caveolin.
Baculovirus constructs encoding either c-Src or v-Src from chicken were the generous gifts of Drs. 2990
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In striking contrast, caveolin and caveolae are reduced or absent in fibroblasts transformed by certain activated oncogenes such as v-Abl or H-Ras G12V 6. Thus, we have termed this amino acid stretch of caveolin residues the caveolin scaffolding domain. These cells express extremely low levels of endogenous caveolin; little or no immunoreactivity was observed with anti-caveolin IgG by Western blotting not shown.
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Deletion mutagenesis indicates that the Src-interacting domain of caveolin is located within residuesa cytosolic membrane-proximal region of caveolin. Please enter a valid postcode.
Thus, it appears that caveolin structurally and functionally interacts with wild-type c-Src via caveolin residues Have one to sell? Learn More – opens in a new window or tab 2990 international postage is paid in part to Pitney Bowes Inc.
The observed differences in the potency of peptide 2 with different Src family tyrosine kinases suggests that i the xs of caveolin with Src family tyrosine kinases is highly specific 291900 ii that caveolin may preferentially interact with a select subset of Src family members in vivo. Portions of the information contained in this table have been provided by carpartsinmotion.
United Kingdom and many other countries See details. Third, it binds to wild-type H-Ras.
Because caveolin preferentially interacts with inactive c-Src but 29109 v-Src and a caveolin-derived peptide or caveolin co-expression can inhibit auto-activation of c-Src, caveolin could act as an allosteric inhibitor of c-Src and other Src family kinases. Insect Sf21 cells were provided by Dr. The subcellular distribution of several signaling molecules is restricted by association with scaffolding proteins Thus, it appears that caveolin prefers the inactive conformation of Src.
To test this hypothesis, we assessed the interaction of caveolin with Src family tyrosine kinases in vitro using i baculovirus-expressed Src proteins c-Src and v-Src and ii bacterially expressed 2190 fusion proteins.
Pdyn (prodynorphin) | Gene Report | BioGPS
The cell pellets were analyzed 29910 both in vitro kinase assay and immunoblotting using anti-Src antibody. The expression of Src tyrosine kinases was monitored by immunoblot analysis with a mAb that recognizes the conserved extreme N-terminal of Src. As we find with caveolin, each of these enzymes is inhibited when bound to AKAP 29 ,